Can we identify a compound that could be added to spermatozoa in vitro Assignment - 1

Can we identify a compound that could be added to spermatozoa in vitro to enhance their motility - Assignment Example In particular, sperm motility is an important parameter because it indicates the integrity of the sperm tail and axoneme structures as well as the status of mitochondrial metabolic machinery (Saharkhiz et al., 2013). The World Health Organization uses a 40% cut off as the lower limit of progressive sperm motility. Although the exact cause of asthenospermia is not clear, there is growing evidence that it may arise due to hyper-viscosity of semen, varicoceles, autoimmunity of the sperm cell, and necrospermia resulting from immotile cilia (Kartagener) syndrome (Saharkhiz et al., 2013). Overall, reduced sperm motility indicates epididymal or testicular failure that can be caused by various factors. Currently, treatment of male infertility has focused mainly on the traditional in vitro fertilization (IVF) approach and newer interventions such as micro-epididymal sperm aspirations and microsurgical fertilization. However, these techniques are uncommon in andrological practice. Current research efforts in this area focus on the use of chemical stimulation of spermatozoa. A number of chemicals can stimulate sperm motility including the addition of compounds known to exhibit phosphodiesterase inhibition (PDEI) activity to enhance sperm motility in vitro without compromising sperm morphology and function. PDEIs are a class of related compounds such as pentoxifylline (PTX) that selectively catalyze the hydrolysis of 3’ phosphate bond in cyclic Adenosine Monophosphate (cAMP) phosphate or cyclic Guanosine Monophosphate (cGMP). In one of the pioneering studies on in vitro stimulation of sperm motility, Shen (1991) demonstrated that Pentoxifylline (PTX) increases motility (velocit y) of ejaculated human spermertozoa both in in vitro aesthenozoospermic samples in oral therapy. Related studies have shown that PTX added in sperm suspensions increases sperm motion within 10 days with the motility characteristics persisting

Management theories and methods in Construction Projects Essay

Management theories and methods in Construction Projects - Essay Example The leader has to lead the team and train them at the same time. A true leader is supposed to find out the root causes of issues and solve them in a way which is thoroughly acceptable to various individuals involved (Nicholas, 1990). In the current scenario, the sub-contractor team is not working properly because of the lack of motivation in them. It is the job of the leader to identify this issue and devise a strategy to motivate the team and boost their morale. Leaders must have high degree of emotional intelligence for identifying the hidden aspects of the issues and for finding out solutions to them. Understanding the mindset of employees and workers is very important because it aids the management in catering for the employees. the needs theory clearly explains how a leader can take control of a scenario and achieve the maximum output. In the current scenario, the mindset of the subcontractor team should be analyzed thoroughly for identifying the problem (Goleman, 1998). Maslows theory of motivation provides the leaders with an easier way of attending to the employees. Since the sub-contractor has gained other contracts with the organization as well, he might have achieved his personal targets already and that is why he is not interested in ensuring quality work. The style theory asserts that managers have different styles of leadership and their employees often respond to each theory differently. The leader in this case first identified the issue, after that there is a simple talk with the sub-contractor in order to help improve on efficiency. As the construction site manager, my responsibility is to convince the sub-contractor about the benefits of working efficiently (Linstead, Fulop & Lilley, 2004). A true leader should be able to convince the employees, workers and sub-ordinates that individual and personal targets can only be achieved if the project is successful. The delays and inefficiency of one team is detrimental for

Twelfth Night and The Winter's Tale Essay Example | Topics and Well Written Essays - 1500 words

Twelfth Night and The Winter's Tale - Essay Example These fools are not used for entertainment purpose alone and suggesting that they were frequently employed by Shakespeare to provide a comedic relief to the audience from dark heaviness of the main action is empty mockery. However, this much is also true that in most Shakespearean plays it seems as if the scenes of clowns are almost strategically placed after particularly violent or dark scenes. The purpose of this essay is to contemplate the role of clowns in two very popular comedies by Shakespeare namely Twelfth Night and The Winter’s Tale to analyze if clowning is employed merely for comic relief or it also works to convey the message of the dramas. Feste is the name of clown who appears in Twelfth Night and is especially favored by Olivia who is the very beautiful countess and has given Feste full authority to cross boundaries and say whatever he likes (LitCharts) understanding his knack for humor and intellect. But, is Feste used by Shakespeare to make jokes alone which have no deep meaning to them or convey no meaning as suggested by some critics that clowns are mainly used to provide comic relief? The answer is a clear no because in the beginning of the play when Olivia is introduced terrifically grieving over her dead brother and displaying no signs of moving on with life, Feste makes a very bold gesture by telling Olivia to quite her mopping attitude which has actually become quite ridiculous. He does not only make silly or shallow remarks about the way she cries or stays melancholic, but illustrates a very important message of life by claiming that it is not him but Olivia who is acting like the real fool wasting all her energy in mourning instead of concentrating on her life. This critically important statement is made by no other character but Feste which suggests that the role of clowns is used by Shakespeare in his plays to both calm the readers and highlight the weaknesses of other characters which are otherwise untouchable and cannot be reproached by anyone. In addition to that, he also thinks that witty criticism is his prized prerogative. So, he is frequently seen driving others mad particularly Olivia’s greedy and wasteful uncle Toby Belch who is one of the favorite targets of Feste. Given the nature of his brilliant criticism and the way he digs with acute intelligence into dark aspects of other serious-natured characters, this much is obvious that Feste is no ordinary fool but an exceptionally smart jester who is a genius with punning. Viola (Cesario), another heroine of the play, describes Feste as the wisest cat in the play. Why would Feste be described as the wisest cat in town were his job cracking jokes alone as assumed by some critics to provide comedic relief from tension of the main plot? In fact, he is described in this manner by others because his intellect of smart wordplay is secret to none and everybody, even those who resent him like Olivia’s uncle, acknowledge the brainpower of thi s enormously powerful Shakespearean clown. He not only picks on the clear weaknesses of others around him, but also has a real talent for shrewdly observing grave or serious situations (Geraghty). He makes people laugh over his wise puns and also makes a good living out of it. He accurately measures the ridiculousness of the starting scene where Olivia is dressed up like a nun for grieving purpose and mockingly characterizes her silly attitude as plain unnecessary and stupid

Porters Management Essay Example | Topics and Well Written Essays - 2500 words

Porters Management - Essay Example This essay stresses that the management styles or the fourth factor that was described by Porter has become very blurred. A dynamic approach is required towards management of organization and it has become imperative to follow a management practice that is sustainable. It has been observed that shareholders have become very demanding and the excessive reliance of the business enterprises on the capital market have made it important for organizations to depend on information in a timely manner. This paper makes a conclusion that the case of national competitive advantage over comparative advantage, an idea created by Porter has both been a source of debate and discussion. A discussion in this topic reveals that the traditional competitive model described by Porter using the developed countries can also be extended to certain other developing countries as well. The case of Ireland, India, Singapore, Korea and Saudi Arabia has revealed that Porter’s model of national competitive advantage is still relevant to explain competitiveness of these nations. All of these nations have developed their competitive advantage over time and provides a thriving climate both for domestic and international businesses. However, there are certain aspects of Porter’s model that is not relevant in present case. For instance, the management styles that have been described by Porter for specific countries are no longer valid in the present organizational structures. The financial cris is of 2008 has revealed that excessive speculation can be extremely dangerous.

Senior Philosophy EXAM 3 Study Guide Essay Example | Topics and Well Written Essays - 1250 words

Senior Philosophy EXAM 3 Study Guide - Essay Example Hence, Epicurus concluded that we should not live in fear of death (Epicurus, 510). 2. According to Dawkins, animal fighting is restrained and gentlemanly in the following way. Animals do not necessarily fight to eliminate their rivals because, by doing that, they would mostly likely be endangering their lives even more. Dawkins argues that in a complex system of rivals, it might be advantageous to your other rivals if you eliminate one of them; your other rivals might stand to gain more from the elimination than you. For this reason, Dawkins argues that animal fighting is, in most instances, based on the advantages that would accrue from the fighting and as a result, animal fighting in most cases are restrained and gentlemanly. 3. Rand claims that Altruism permits no concept of self-respecting, self-supporting man, and no concept of justice. To support her claim, Rand contend that Altruism as an ethical theory is based on the false idea that, anything that is good for an individual( for selfish interest) is morally bad, while what is good for other people, not an individual, is morally right. For this reason, Rand argued that Altruism has no room for people first fulfil their own interests before catering for the interests of other people. For this reason, Rand claims that Altruism is against the virtues of self-respect, self-supporting, and the virtue of justice. 4. In his account of happiness in the Nicomachean Ethics, Aristotle makes it clear that happiness is not merely a feeling or a sensation. For Aristotle, happiness is the highest and final good for all activities of man. As such, any man’s activity is aimed at attaining happiness for man. Happiness, therefore, is the final end of all human being’s endeavour and human beings seek happiness for its own sake. All the other good that human beings seek, for instance pleasure and honour, may be sought partially for their own sake, but ultimately they will be sought for the sake of happiness. F or that reason, happiness cannot be sensation or feeling because feelings and sensations cannot be the final end, pleasurable feelings are sought for the sake of happiness, which is the highest good. Hence, happiness is not simply a feeling or a sensation. 5. According to Immanuel Kant, it is morally wrong for one to take a loan and promise to repay it, while one knows that they will not be able to repay it. To support his claim, Kant used his moral maxim, i.e. the categorical imperative to judge whether or not such an action is morally justifiable. The categorical imperative states that, in moral dilemma, you should always act in a manner that you would wish your manner of acting to be made a universal law, applicable to all similar cases. Kant, therefore, argued that since one would not wish breaking promises to be made into a universal law, it is, therefore, morally wrong for one to fail to repay a loan that they had promised to repay. 6. According to Mill, one can tell whether o ne pleasure is of greater quality than another by the use of the following criteria. If people are asked to choose between two pleasures, pleasures of which they all have prior experience, the pleasure that majority of people will choose, not due to moral obligations or for any other reason, but for the sake of the pleasure itself, then that pleasure that is proffered by the majority of people is of higher quality than the other pleasure that was preferred by only a

Circular Dichroism and Secondary Structure of Proteins Essay Example for Free

Circular Dichroism and Secondary Structure of Proteins Essay Proteins are vital to an organism life; they are involved in nearly all cellular functions. It is an essential part of enzymes, the cellular membrane, active transport, protein synthesis and wound healing. Because one relies so heavily upon proteins and its function the structure of proteins is also very important. â€Å"The way a protein will fold over its self-determines how it interacts with other chemicals in its proximity, primarily because of different attractive forces being subjected at specific angles from certain amino acids on the protein’s primary structure and the final shape in the tertiary and quandary structure (Circular dichroism ). † The structure of proteins can range from simple to complex molecules. Proteins may consist of a primary, secondary, tertiary, and quaternary structure. The secondary structure consists of hydrogen bonds which join amide and carboxyl groups. These bonds aren’t far from the backbone of proteins. This structure is capable of structures such as alpha helicies, beta sheets, and beta turns (Jim, 2007). The physical, secondary structure is important because it helps in determining the activity of a protein. Circular Dichrosim spectroscopy has been identified as prevalent application used in structural biology in determining whether a protein is folded, characterizing its secondary structure, tertiary structure, and the structural family along with other uses as well (Circular dichroism ). Circular dichroism, CD spectroscopy has defined a form of light absorption spectroscopy. It measures the difference in the absorption of circular polarized light by a substance on the right and left. The secondary structure of a protein can be analyzed between the spectrum of approximately 260 and 180 nm. Estimates of secondary proteins can be compared to X-ray crystallography or NMR (Kelly, Jess, C., 2005).The structures identified in this spectrum are the alpha helix, parallel and antiparallel beta sheet, and turns (Berndt, 1996). The only drawback with CD is that even with the implied spectrum, it has been found that there is no exact standard reference spectrum for a pure secondary structure. Synthetic homopolypeptides used to obtain reference spectra are in general, poor models for the secondary structures found in proteins (Berndt, 1996). The CD signal reflect the entire molecular population; it can determines how much of a certain structure and protein contains. It cannot determine the specific residues involved in the alpha-helical portion. In Circular Dichroism a linear polarized light passes through a optically active sample of a protein. This protein has a different absorbance for components. The amplitude of the stronger absorbed component will smaller than that of the less absorbed component. A projection is created of the resulting amplitude. The result is no longer a linear line but and ellipse (PARTHASARATHY, 1985). Different analyses have been developed to help with various contributions that arise from the different types of secondary structures present in a single molecule. The use of reference spectra have been created from known protein structures to help find the overall and secondary structure of unknown proteins (Whitmore A., 2007). â€Å"Recently a new reference dataset of SRCD spectra of proteins of known structure, designed to cover secondary structure and fold space (Berndt, 1996).† Works Cited Berndt, K. D. (1996, May 31). 4.2.1 Circular dichroism spectroscopy. Retrieved October 02, 2012, from 4.2.1 Circular dichroism spectroscopy Circular dichroism . (n.d.). Retrieved October 02, 2012, from APlab: http://www.ap-lab.com/circular_dichroism.htm Jim, C. (2007, August). The Structure of Proteins. Retrieved October 02, 2012, from Chemguide: http://www.chemguide.co.uk/organicprops/aminoacids/proteinstruct.html Kelly, S. M., Jess, T. J., C., P. N. (2005). How to study proteins by circular dichroism. Biochimica et Biophysica Acta 1751, 119 – 139. PARTHASARATHY, M. (1985). Protein secondary structure from circular dichroism spectra. Proc. Int. Symp. Biomol. Struct. Interactions, 141-149. Whitmore, L., A., W. B. (2007). Protein Secondary Structure Analyses from Circular Dichroism. Biopolymers, 392-400. `

Effect of Globalisation on Spatial Patterns of Economy

Effect of Globalisation on Spatial Patterns of Economy Globalisation and the spatial structure of the economy: Critically discuss how changes related with globalisation can affect cities and the spatial patterns of economic activities? Globalisation has become one of the key concepts in the social sciences at the turn of the twentieth century. A term that has come to be used globally which relates to the processes of social relations acquiring relatively distance-less and borderless qualities that place can be argued as a homogenous landscape (Scholte, 1997). However as Julius (1997) finds there is confusion about globalisation continuing within the disagreement over whether it is â€Å"a process or an end-state†. Globalisation can be seen as the world-wide interconnectedness between nation-states. In which social arrangements such as power, culture, politics and norms become intangible from their spatial context due to the acceleration, diffusion and expansion of transnational flows of people, goods, finances and information (Berkens, 2004).Globalisation can be seen as essentially a process driven by economic forces, having profound effects on cities and the economy (Sideri, 1997). Globalisation can be argued as a more recent expansion of the previous emerging relationship between urbanisation, industrialisation and economic development which initially began in Western Europe in the late fourteenth century; associated with the development of European wide trade links (McCann, 2013). However the concept can date as far back to Colonialism and the colonial power of Europe. The first era beginning with the arrival of Christopher Columbus in America (1492) combined with the expansion of the Spanish and Portuguese empires (Friedman, 2007). This westward colonial expansion of Spain to America after 1492 proceeded the sixteenth century with the transatlantic trade links; the first economic global expansion for European countries, the development of overseas empires in non-adjacent regions (McCann, 2013). This concept enabled globalisation to be defined as an economic structure whilst being a political phenomenon, shaped by negotiations and interactions between institu tions of transnational capital and nation states (Guttal, 2007). Dating Globalisation back to the colonisation of Asia, Africa and America alongside the search for new markets and sources of wealth fanned by the industrial revolution; resulted in the production of international commodity markets and mercantilist trade (Guttal, 2007). By 1800 Britain was argued as most industrialised country in the world due the enhancement of the industrial revolution. London was seen as the second largest city in the world and some 57% larger than Western Europe’s second largest city (McCann, 2013). Due to the establishment of colonies and industrial trade links since the Second World War much of the world has become more interlinked through innovations and advances within transportation, communication and information technology (Guttal, 2007). During the 1980’s the development of engineering techniques and technologies facilitated the reduction in skilled labour and movement towards machinery within batch production. Variety and availability being the main drivers of these advancements resulted in an alteration of the economy; a reduction of jobs and movement of manufacturing out of the city to suburb locations (Duffy, 1995). Furthermore labour intensive sectors located in innermost locations such as clothing and tobacco have been outsourced overseas to cheaper labour intensive factories in order to maximise profit. The new jobs created will need white collar workers and will require meeting the need of the consumer, so location needs to optimise their competitive advantage (Duffy, 1995). By the latter of the twentieth century there was an unprecedented increase in economic capacities, power and reach of private corporations which many were operating transnational. New York was seen as the principal city whilst all cities had become the economic engines increasing in size and increasingly interlinked (McCann, 2013). Globalisation has been as a result of modernisation and capitalist expansion, engaging the incorporation of all economic movement into a â€Å"global† homogenous market place. Materialisation of this integration is due to the collapse of nation barriers to external trade and finance centres, deregulation of the economy, expert driven economic growth and the facilitation of free market capitalism (Guttal, 2007). The modern structure of regions has enabled regional organisations to combine highly contrasting elements of zones of abandonment, with areas of primary resources whilst comparing and contrasting the reurban/suburban areas to the outlook of the urban zones (Claval, 1998). In the past decade the notion of globalisation yielded numerous pieces of literature and has led to widespread debates over the explanation. Various disciplines have deliberated the subject area merely concluding that the concept involves change whilst new arrangements are emerging that differs from those in previous times (Berkens, 2004). This notion of change has altered cities and economies spatially. Globalisation has led to the homogenisation of consumer tastes, the amalgamation and expansion of corporate power, increases in wealth and poverty, the â€Å"McDonalisation† of food and culture and the growing of democratic ideas (Guttal, 2007). All of which have caused the outsourcing of production overseas. Trans National corporations (TNCs) today are seen as the main drivers of globalisation; they are the primary beneficiaries of international trade and the most powerful promoter of liberalisation, deregulation and privatisation of production. TNCs are global interconnected businesses which segment the manufacturing process, due to collapse of trade barriers and global regulations (Buckley and Ghauri, 2004). This outsourcing multiple partial operation approach of production combined with the development of cheap transportation and communication networks has allowed an increasing division of production globally. This spatial dispersion of economic activity (Sassen, 2001) has allowed for TNC’s to outsource manufacturing to subtracting independents many of which are located within South East Asia and Mexico. This outsourcing approach allows for companies to meet the demand of the consumer induced population whilst maintaining profits and cheap transportation and communication costs (Claval, 1998). This reorganisation of production overseas resulted in the expansion of international trade and the integration of financial markets. The expansion and spatial dispersion of economic activity has brought about capital mobility. This reorganisation of the financial industry has been typified by the sharp growth production of innovation and multiplication of financial firms whilst shifting the industry away from American transnational banks to major hubs of finance. The integration of markets being a defining characteristic of globalisation (Buckley and Ghauri, 2004) has enabled the integration of local markets into regional trading systems which obtain continental links as a result of the overseas journey of innovation. This spatial change of economic activity from the movement of jobs from highly developed areas to less developed low wage locations has enabled capital mobility. This is not only comprised spatially but through the technologies that provide capital mobility and by maintenance of the decentralised global production system (Sassen, 1988). This increased mobility of capital has altered the geographic or ganisation of production and the network of financial markets whilst reorganising the employment structure. Financial markets goods, services or labour are all moving towards social integration. This change has meant no individual national capital market can have a sustainable independent existence. Regional economic integration is becoming increasingly efficient in integrating goods and services whereas labour markets are functionally separate at national level and integration being resisted by national governments (Buckley et al, 2001). Due to this the largest multi-national enterprises can exploit these differences within the markets to maximise profits (Buckley and Ghauri, 2004). The increase in volume of financial industries (Sassen, 2001) has given major cities a key role in the management and control of such global network. The importance of centralisation has enabled cities like New York, London and Tokyo to become centres of finance and global servicing. All have contributed to the increase of centralised services for management and regulation of the economy accelerating transactions globally (Sassen, 2001). This increased shift of economic activity to finance and highly specialised zones has altered management within companies from an activity focussed on production to one that is financially focussed. The involvements of the complexity of transactions lead to the array of specialised services leading to the high densities and agglomeration economies. This continuous building of high rise office complexes accelerates land price triggering a competition for land. This process of agglomeration represents a phase in the formation and expansion of an industrial complex controlled by command functions and finance; whilst referring to benefits that accumulate when firms locate in â€Å"propinquity† to one another (Bingham Mier, 1993). Alfred Marshall a leading economist observed that firms often continued to cluster successfully in the same locations because of knowledge â€Å"spillovers†, local and non-traded inputs and a local skilled labour pool (McCann, 2013). Marshalls’ model looks at how individuals acquire skills by interacting with one another with denser areas increasing in rate; with the strength of the economy rising there is a higher mean and variance of skills (Glaeser, 1999). The integration of local and national economies into an agglomeration whilst unchanged by protectionism allow for economic grow th. Wealth will be created and shared with more people to enjoy the benefits of modernisation, technological advancements and society. However critics may argue that globalisation has resulted in the clustering of the affluent and educated class whilst fracturing working classes and marginalising the poor who may not have the skills and economic asset to gain from an open market approach (Guttal, 2007). The agglomeration approach gives sense to global cities; bases of spatial organisation and communication of production and markets (Duffy, 1995). On a smaller scale â€Å"second cities† such as Birmingham have consolidated position as the source of services for businesses. The process allows similar or like economies and industries to cluster together in order to raise price, competition and consumers to raise their economic profile. Allowing benefits from reduced transport and negotiating costs, pool of labour and the sharing of speciality machinery (Bingham Mier, 1993). This competition for inner city location can be monitored by the Bid Rent model by Alonso, 1964 (McCann, 2013). However once a centre of agglomeration has occurred it becomes difficult for institutions to locate outside of the area due to the profound benefits and links that have evolved, however FDI may offer a solution. Within the recent global economy the emphasis of new investment and transactions is centred by Foreign Direct Investment (FDI); the allowance to inquire a firm in a foreign country (Sassen, 2000). In recent years the internationalisation of production to developing economies has been the main focus. Throughout the 1990’s developing countries had a lower investment flow than developed countries; it was still observed as high in historic terms, hence reflecting the growing internationalisation of economic activity (Sassen, 2000). Expansion of economic growth driven by the outsourcing and investment of primarily manufacturing has enabled areas such as China to grow and become a global economic driver. The urban economic agglomeration which occurs throughout China has enabled itself to be an economic superpower (Chow, 2002). The rise of China has enabled a manufacturing, production and service base for a labour intensive industry. The optimum location can thrive off a growing economy with the ability to absorb raw materials whilst having in exhaustible cheap labour for production and a growing internal market. Since 2003, China has been the main location of Asian exports and served as the primary incentive of growth within the world’s economy over the past decade (Guttal, 2007). The more recent approach of The Asian Tiger economies has led to the independence of Hong Kong, Taiwan, Singapore and South Korea. By the twenty-first century all four nations had seen advancements towards high income economies, specialising in aspects of competition. This enhancement altered their global economic structure; changing from traditional, agriculturally based societies to rapidly growing NIC’s with economic centres (Davies and Gonzalez, 2003). An expansion of these economies led to the snowball effect of â€Å"second-tier† East Asian developing economies; the emergence of Indonesia, Malaysia and Thailand. With the resurgence of Asia being the most significant global shift; becoming economic focuses within the spatial economy (Dicken, 2007). The agglomeration of local goods and services enables the â€Å"death of distance† (Cairncross, 1997), the reduction of friction of services between goods and individuals. This Global process does not solely just affect economic activities it affects the social and population structure of a city. Due to the diversification of cultures and norms across globalisation, population groups can agglomerate together. The creative class brings innovative new ideas to create change (Clifton, 2008). This innovation of clustering has enabled creative connectivity and the ability of creative individuals to push forwards to the frontiers of research within MNE’s. This area of potential allows the integration of individuals (Antonelli, 2000). It has been noted by academics that population loss within a city can occur due to globalisation; largest decrease in inner city areas (Duffy, 1995). Housing and workplaces were cleared in replace of lower income density housing and public amenities. However the opposite occurred in other locations known as counter-urbanisation. Within the core of the city homes and amenities where produced to attract the middle class back into the city, with the attempt of inner city redevelopment. Thus as a result of globalisation a snowball effect happened with the migration of the population which in turn led to redevelopment of the inner city. As Duffy (1995) states a city shall strive higher if there is a mix of population and norms as opposed to a uniform homogenous landscape. Whilst Glaeser identifies that high skilled and high income workers migrate back to city centres as a result of re-urbanisation as part of the consumer city hypothesis (McCann, 2013). Nonetheless there are challenges in which affect regions, whilst large markets offer investors great liquidity and competitive prices of stocks and greater security. Agglomerations of economic power in major metropolitan regions cause the peripheral areas of regions to be plundered for their raw materials and inexpensive unskilled labour intensive rolls. This economic clustering causes greater income inequalities whilst destroying the linkages of local communities and the homogenisation culture of western values (Buckley and Ghauri, 2004). However Rugman (2000) found that it is hard to address these issues because they hit directly at the logistics of a capitalist approach. For example due to the dynamics of the market system old jobs are destroyed whilst new jobs are being created and as the process escalates jobs become ever more insecure (Burchell et al, 2005). In conclusion the outsourcing and global expansion approach offers profound effects on cities and economic activities. Cities which were once industrialising hubs have lost the capital from old industrial centres and have transformed into the inflow of capital into NIC’s and TNC’s (Sassen, 2001). Globalisation has allowed for the development of physical and technological infrastructure enabling global linkages and the share of economic activity through the internet being the backbone of society (Castell, 2001). The interconnectedness of cultures and norms through the realms of trade offers endless benefits and negatives throughout spatial economies and cities. Development of economies can be modelled using regional econometric models which look at economic linkages that exist within and between regions worldwide. The model can predict that an increase in regional wage can have a long term effect on the population balance through in-migration; occurring whenever the regi onal wage rises relative to the national wage (Armstrong Taylor, 2000). Models allow prediction of the affects globalisation has spatially allowing analysis and discussion. Reference List Antonelli, C. (2000) Restructuring and innovation in long-term regional change, Oxford University Press, Oxford, pp.373-394. Armstrong, H. and Taylor, J. (2000) Regional Economics and Policy, 3rd Ed. Blackwell Publishing, Oxford, 437. Berkens, H.J.J.G. (2004) Global Opportunities and Institutional Embeddedness, UNITISK, Czech Republic Bingham, R.D. and Mier, R. (1993) theories of local economic development: perspecvtives across the discipline, SAGE publications, California, 319 Buckley, P.J., Clegg, J., Forsans, N. and Reilly, K.T. (2001) Increasing the size of the ‘country: regional economic integration and foreign direct investment in a globalised world economy’, Management International Review, vol.41, issue 3, pp. 251-274. Buckley, P.J. and Ghauri, P.N. (2004) Globalisation, Economic Geography and the Strategy of Multinational Enterprises, Journal of International Business Studies, vol. 35, no. 2, pp. 81-98 Burchell, B., Ladipo, D. and Wilkinson, F. (2005) Job Insecurity and Work Intensification, Routledge, London New York, pp.240 Cairncross, F. (1997) The Death of Distance: How the Communications Revolution will change our lives, Harvard Business School Press, Boston Chow, C.G (2002) Chinas economic transformation, Blackwell, Oxford Claval, P. (1998) An Introduction to Regional Geography, Blackwell Publisher, pp.299 Clifton, N. (2008) The â€Å"Creative Class† In the UK: An initial Analysis, Geografiska Annaler: Series B, Human Geography, vol. 90, issue1, pp.63–82. Davies, J.C. and Gonzalez, J.G. (2003) Scholarly Journal Articles about the Asian Tiger Economies: Authors, Journals and Research Fields, 1986-2001, Asian-Pacific Economic Literature, vol.17, issue 2, pp.51-61. Dicken, P. (2007) Global Shift: Mapping the Changing Contours of the World Economy, 5th Ed. SAGE publications ltd, London pp.599 Duffy, H. (1995) Competitive Global cities: Succeeding in the Global Economy, Chapman Hall, London, 195. Freidman, T, L. (2007) The world is flat: A brief history of the Twentieth Century, (3rd Ed.), Picador, New York Glaeser, E.L. (1999) Learning in Cities, Journal of Urban Economics, vol.46, issue 2, pp. 254-277. Guttal, S. (2007) Globalisation. Development in practice, vol.17, no.4/5, pp.523-531 Julius, D. (1997) Globalisation and Stakeholder Conflicts: A Corporate Perspective, International Affairs, vol. 73, no.3, pp. 453-468 Scholte, J.A. (1997) Global Capitalism and the state, International affairs, vol. 73, no.3, pp.427-452 Sassen, S. (1988) The mobility of labour and capital: A study in international investment and labour flow. London: Cambridge university press Sassen, S. (2000) Cities in a world economy 2nd Ed. Pine forge Press, California, 182. Sassen, S. (2001) The Global City: New York, London, Tokyo, 2nd ed, Princeton University Press, pp.447. Sideri, S. (1997) Globalisation and regional integration, European Journal of Development Research, vol 9, issue 1, pp. 38-81. NEED McCann 2013 Rugman 2000 Castell 2001 Page 1 of 11